Determinación del medio de cultivo para el crecimiento y esporulación de sarocladium oryzae (sawada) gams & hawks
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Abstract
Sheath rot diseases is caused by S. oryzae detected for the first time in Cuba by the end of 1997. This fungus and the mite Steneotarsonemus spinki produced serious damage in Pinar del Río and La Habana provinces. Biological and epidemiological studies on this pathogen are very scarce so it is necessary to carry out different biological studies to our S. oryzae isolates. Growth and sporulation were assessed on five culture media in order to select the best one. The media studied were: modified Czapek-Dox Agar, Rice Agar, Sabouraud’s Agar, Malt Extract Agar and Patato Dextrose Agar. The micelial growth and sporulation were measured at 3, 6, 9 and 15 days. Colonies were described for every culture media having differences in relation to their color, shape and texture. The best media when assessed at 15 days were Malt Extract Agar reading 51 mm the colony diameter, followed by Czapek-Dox reading 45 mm and on Sabouraud’s Agar colonies reached 41 mm mycelial growth. The sporulation value reached on Sabouraud’s Agar was 1.4 x 107 conidio/ml. For the rest of media, the sporulation ranged from 1.1-1.4 x 106 conidia/ml. Czapek-Dox culture medium was selected as the proper one for growth, sporulation and for diagnostic purpose since orange pigment in colony reverse, appeared when cultured on it.
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References
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