Distribution of Tomato chlorotic spot virus (TCSV) in tomato crops of tomato (Solanum lycopersicum L.) in Cuba
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Abstract
In Cuba, since 2009, symptoms on tomato plants (Solanum lycopersicum L.), similar to those caused by the genus Tospovirus, were observed. However, since the 2012-2013 campaign, there was a considerable increase on symptoms intensity, in some crop areas of Artemisa, Mayabeque and Guantanamo provinces; and the presence of Tomato chlorotic spot virus (TCSV), belonging to the genus Tospovirus, was determined by molecular techniques. The aim of this study was to determine the presence of this virus as well as the symptoms it cause, in tomato growing areas of the remaining provinces. For this purpose, areas of different municipalities of the western, central, eastern provinces, and protected cultivation houses were sampled. The samples was mechanically inoculated in tomato var. Rilia and in indicator plants Nicotiana glutinosa L., Nicotiana tabacum L. var. White Burley, var. Criollo 98 and Chenopodium quinoa Wild. To confirm the presence of Tospovirus, and species, samples showing symptoms were analyzed by RT- PCR Hypersensitivity reactions were detected on indicator plants. The genus Tospovirus and species TCSV were detected in all sampled locations, except for some samples from Guantanamo province, with which the segment was not found with the expected hope for the pair of primers in RT-PCR. In tomato, it was observed, in general, leaves displaying chlorotic and necrotic spots, bronzed coloration, and fruits with very pronounced necrotic spots and rings.
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